Expansion Microscopy:

Abstract

Fluorescence microscopy has become an essential tool for biological and translational research; however, the diffraction limit of light limits our ability to resolve nanoscale cellular architectures. Super-resolution approaches have been developed to overcome this barrier, and expansion microscopy (ExM) is among the most recent, achieving nanometer- to angstrom-scale imaging through a unique strategy: physically expanding the specimen itself rather than pushing the optics.

We have developed 3D-ExM, an economical, reproducible, and user-friendly method that achieves isotropic three-dimensional expansion without environmental control or additional instrumentation.

In this seminar, I will describe the 3D-ExM workflow, evaluate its performance, and discuss how it can be extended to new biological questions.